Figure 7.

IL-13 induces EGFR phosphorylation and enhances airway epithelial repair via HB-EGF. Confluent monolayers of 1HAEo⁻ cells were treated with IL-13 (10 ng/ml) with or without concurrent treatment of anti–HB-EGF (3 μg/ml). (A) Total protein lysates were collected and phosphorylation of EGFR was examined by Western blotting. Injured monolayers of 1HAEo⁻ cells were treated with IL-13 (10 ng/ml) with and without AG1478 (1 μM), anti–HB-EGF (3 μg/ml), and GM6001 (50 μM) (B). Corresponding wound areas were determined at 0, 8, and 24 hours after injury. Data are mean ± SEM for eight wounds in each group. *P < 0.05, ^‡P < 0.01.