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. 1999 Jul 6;96(14):7831–7836. doi: 10.1073/pnas.96.14.7831

Figure 3.

Figure 3

Partial NMR spectra of purified AOB-CoA obtained from ACMR-catalyzed conversion of AB-CoA with NADH and O2; variation of labeling patterns. (A) 13C decoupled 1H NMR spectrum of AOB-CoA obtained from conversions of [ring-13C6]AB-CoA with NADH in H2O (buffer). (B) 1H NMR spectrum of AOB-CoA from [5-2H]AB-CoA and NADH in H2O. (C) 2H decoupled 1H NMR spectrum of sample described in B. (D) 2H decoupled 1H NMR spectrum of AOB-CoA from AB-CoA and [4R-2H]NAD2H in H2O. (E) 2H decoupled 1H NMR spectrum of AOB-CoA from AB-CoA and NADH in 2H2O. 1H coupling patterns are indicated above the signals. Signals resulting from 2H labeled isotopomers of AOB-CoA are indicated. For numbering, see Fig. 4 legend.