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Injection of N-cad mRNA does not correct the NTD in MZoep mutants
| Pineal morphology | ||||
| Embryo | Dose of N-cad mRNA (pg) | Round | Expanded | Divided |
| WT | 0 | 75 | 0 | 0 |
| WT | 1.9–75 | 37 | 0 | 0 |
| MZoepm134 | 0 | 0 | 1 | 29 |
| MZoepm134 | 0.6–7.5 | 2 | 11 | 58 |
| MZoepm134 | 25–75 | 1 | 1 | 33 |
Embryos were fixed at approximately 1 dpf and processed for whole mount in situ hybridization with an antisense probe to otx5.
