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. 2007 Oct 23;111(3):1234–1239. doi: 10.1182/blood-2007-09-112680

Figure 2.

Figure 2

Western blot of constructs 1 to 4 and platelet lysate (Pl) using monoclonals AP3, AP5, and anti-V5 for detection. Constructs 1 to 4 migrated as expected on the basis of their predicted molecular weights and reacted with the appropriate probes. As expected, construct 4 containing mutations A50S, R62Q, and D66A was not recognized by monoclonal AP3, and platelet GPIIIa (Pl) was not recognized by anti-V5.