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GS was pulled down with GST-glycogenin—GST-GN(297–333)—fusion protein before and after α-amylase digestion of glycogen in skeletal muscle extracts from wild type (RGL WT) and RGL knock-in homozygous (RGL kin hom) mice. (A) GST-GN(297–333) pulls down almost all GS, but (B) neither the WT nor the truncated RGL (RGL trunc) was pulled down by GST-GN(297–333).
