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Western blotting of high-speed supernatants and pellets of skeletal muscle extracts from WT, RGL knock-in heterozygous (het), and RGL knock-in homozygous (hom) mice. High speed (HS) ultracentrifugation at 100,0000g for 90 min was used to pellet glycogen. Western blots for RGL, GS, and GP were then performed on the supernatant and pellet fractions.
