Figure 1.

Immunoblotting analysis of native and mutant GP Ibα amino-terminal domain fragments. Recombinant fragments with either normal sequence (WT) or the G233V mutation were analyzed after ammonium sulfate precipitation from culture medium and partial purification by size-exclusion column chromatography. Each fraction obtained from the chromatography separation was evaluated for reactivity with the anti-GP Ibα monoclonal antibody LJ-Ibα1 after SDS/PAGE and protein transfer to nitrocellulose. Positive bands were visualized with rabbit anti-mouse IgG labeled with ¹²⁵I and autoradiography.