Figure 7.

Time course of individual interactions between latex beads coated with GP Ibα amino-terminal domain fragments and immobilized vWF. The beads used in these experiments, coated with normal (WT) or mutant (G233V) GP Ibα fragment, were prepared and perfused at a 1,300-s⁻¹ wall shear rate as described in the legend of Fig. 5. The duration of contact of each individual bead rolling on immobilized vWF is presented here as a distribution in arbitrary categories. Only beads that could be followed from the beginning of interaction with the surface were considered. The percentage of beads in each category was calculated relative to the total number of beads interacting with the surface. The Insets show selected results with a reduced scale on the ordinate to provide a better representation of the lower values in the categories reflecting longer duration of contact with the surface.