Figure 1. Lack of Spermatogonia in Nonphosphorylatable separase S1121A Mutant Mice.

(A) Testes from 6-wk-old control and mutant littermates.

(B) Micrographs of hematoxylin and eosin–stained sections of testes in (A).

(C) Micrographs of hematoxylin and eosin–stained sections of testes isolated from animals at different ages.

(D) Immunofluorescence staining of Tra98 (red) and Sox-9 (green). Sections of 4-d-old testes were used. Nuclei were counterstained with DAPI (blue).

(E) Western blot analysis of Tra98 and Sox-9 using 2-wk-old testes.