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. Author manuscript; available in PMC: 2009 Jan 20.
Published in final edited form as: Virology. 2007 Oct 15;370(2):310–322. doi: 10.1016/j.virol.2007.08.038

Figure 5.

Figure 5

Generation of memory LLO91−99 –specific CD8+ T cell responses in LCMV-infected mice. (A) Experimental design. BALB/c PKO and BALB/c WT mice were infected with 2×105 PFU of LCMV Arm i.p. and 43 days later were infected with 3×106 CFU of Att LM. CD8+ T cell responses were then analyzed at the indicated time points after infection by LLO91−99 –stimulated ICS. (B) Representative dot plots from infected mice. Numbers represent the percentage of CD8+ T cells that produce IFN-γ after incubation with (top number) or without (lower number) LLO91−99. Mean, +/− SEM, total number of LLO91−99 – specific CD8+ T cells/spleen of 4−5 mice/group in mice that were (C) infected with LCMV or (D) were not infected with LCMV. Data was pooled from 2 individual experiments. Dashed line indicates the LOD. (E) The mean (+/− SD) number of bacteria per spleen was determined 5, 7, 15 days after LM infection in BALB/c PKO and BALB/c WT mice infected with LCMV 43 days prior to LM infection. Numbers indicate mice/group with detectable LM loads (n = 3 mice / group). Dashed line indicates the limit of detection (LOD).