Centroid analyses of 2-DG uptake support a ventral shift of glomerular activation with increasing carbon chain length. A: The lateral part (top portion) of the glomerular region outlined in Fig. 1A was included in this centroid analysis, and the average centroid position along the dorsal-ventral axis was plotted for each alkane. Increases in the coordinates indicate increasingly ventral positions. Standard errors are represented here. As appeared to be the case from inspecting the 2-DG uptake patterns, centroids shifted ventrally from octane (C8) to tetradecane (C14), and became more dorsal again with pentadecane (C15) and hexadecane (C16). The observed centroid progression was found to be statistically significant (ANOVA, p < 0.001). B: The analyses described in A were applied to the medial aspect (bottom portion) of the outline (Fig. 1A), and results were similar to those observed in A (p < 0.001). In this panel, increasingly ventral positions are represented by smaller coordinates.