Figure 1. Examination of CPC presence in cultures of differentiating mES cells temporally (qRT-PCR).

Relative RNA levels of genes associated with cardiac differentiation in EBs as measured by qRT-PCR. Brachyury expression is associated with mesoderm formation. The transcription factors Nkx2-5 and Tbx5 are indicators of initiation of cardiac development, and the structural proteins Myh6 and Tnnt2 are expressed in functional cardiomyocytes. Error bars indicate SD.