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. 2008 Feb 1;118(3):894–903. doi: 10.1172/JCI33942

Figure 5. Proliferation and clonal capacity of mES cell–derived CPCs.

Figure 5

(A–D) Immunocytochemical analysis of FACS-sorted GFP+ CPCs isolated on differentiation day 5 and cultured in suspension as aggregates for 10 days in the presence of mouse recombinant Igf1. Nkx2-5 (A and B) or GFP (C and D; red) were detected in cells expressing the proliferation markers Ki-67 (A and C) or Pcna (B and D; green). Nuclei were stained with DAPI (blue). (E–H) Clonal capacity of mES cell–derived CPCs. Sorted GFP+ CPCs were plated on mitotically inactivated MEFs at a low plating ratio. Within 4 days after plating, GFP+ colonies were observed (E and F). The colonies grew in size and were either further passaged or allowed to differentiate (G and H). Panels E and G are bright-field images corresponding to F and H, respectively. Scale bars: 20 μm.