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Coimmunoprecipitation of Rb by topo IIα-specific antibody in total extracts of Rh1 cells. Immunoprecipitations (IP) were performed with antibodies to the C-terminal region of topo IIα (Ab-1), Rb (G3–245), and E2F-1 (KH-95) or rabbit IgG. The immunoblots represent three separate experiments. (Top) Blotted with Ab-284. (Middle) Blotted with G3–245. (Bottom) Blotted with KH-95. Lanes 1–4, IP with the indicated antibodies; lane 5, direct immunoblots of the cell lysate used in IP.
