Figure 3.

Amino acid sequence comparisons for MMOB and related hydroxylase cofactor proteins. MMOB proteins labeled B-caps, B-trich, and B-st.M are from M. capsulatus (Bath), Methylosinus trichosporium, and Methylocystis sp. strain M. Cofactor proteins for the toluene monooxygenases systems include TMOD, protein D of the toluene-4-monooxygenase system from Pseudomonas mendocina; T3MOD, cofactor protein of the toluene-3-monooxygenase system from Pseudomonas picketti; and TbmC, cofactor protein of the toluene/benzene-2-monooxygenase system from Pseudomonas sp. CF600. The two phenol hydroxylase cofactor proteins include PH3_ac of Acinetobacter calcoaceticus and P2 from Pseudomonas putida. Helices and β-strands of MMOB (B-caps) are provided and labeled above the primary sequence as arrows and bars, respectively. Residues conserved in at least six of the seven sequences are shaded cyan, while those with conservative mutations are shaded purple. Remaining residues that are identical in all MMOB species are shaded gray. This alignment is described in more detail elsewhere (4).