Figure 3.

Concentration dependence of the renaturation of pressure-denatured complex. The experiment of Fig. 1b was repeated at different complex concentrations, and the observed rates from the double exponential analysis are plotted (open circles). For the concentration dependence of the association rates of the free fragments in equimolar concentrations at atmospheric pressure, the fragments were mixed at the concentrations indicated in 50 mM [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)methane (Bistris) buffer, pH 6.0. The fluorescence intensity of the tryptophan probe was monitored, the data fitted by using a double-exponential equation, and the observed rates plotted (closed circles). The second-order rate constants were estimated from the initial slopes.