Table 2. Model parameters.

Parameters	Comments and References
Species initial concentration
[Cdc20] =  2.2* 10−7M	[40], [55], [71]
[Mad2]total =  2* 10−7M	[40], [55], [71]
[BubR1:Bub3] =  1.3* 10−7M	[40], [55]
[APC] =  0.9*10−7M	[69]
Other species are zero
Species concentration ratios
25% of [Mad2]total associated with Mad1, [Mad1:C-Mad2] = 25%[Mad2]total	[33], [40]
[O-Mad2] = 75%[Mad2]total	[40], [71]
Model–Parameters
k1 = 2*105 M−1s−1	[31]
k-1 = 2*10−1 s−1	[31]
K2 = 108 M−1s−1	[24]
K3 = 10−2 s−1	[24]
K4 = 107 M−1s−1	[62]
k-4 = 10−2 s−1	[62]
K5 = 104 M−1s−1	[62]
k-5 = 10−1 s−1	[62]
K6 = 103 M−1s−1	[15]
K7 = 108 M−1s−1	This study
k-7 = 8*10−2 s−1	This study
K8 = 5*106 M−1s−1	This study
k-8 = 8*10−2 s−1	This study