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Purification of recombinant ACCO
| Purification step | Total protein, mg | Total activity, nmol C2H4/min | Specific activity, nmol C2H4/min per mg protein | Activity recovery, % | Purification, fold |
|---|---|---|---|---|---|
| Lysate | 9,262 | 96,320 | 10.4 | 100 | 1 |
| Q-Sepharose | 1,558 | 71,028 | 45.6 | 73.7 | 4.4 |
| Phenyl-Sepharose | 452 | 33,208 | 73.4 | 34.5 | 7.1 |
| Superdex-75 | 370 | 28,176 | 75.8 | 29.3 | 7.3 |
Cell pellet (100 g) was lysed and recombinant ACCO was purified as described in Materials and Methods.
