Figure 5.
Structure of siRNA expression plasmids. The GMD siRNA expression plasmid consisted of a puromycin resistance gene and a short hairpin siRNA expression cassette controlled by the human U6 promoter (A). FUT8 siRNA expression plasmids consisted of a puromycin or hygromycin resistance gene and a short hairpin siRNA expression cassette controlled by the human tRNAval promoter (B or C). The siRNA tandem expression plasmid consisted of a hygromycin resistance gene and two short hairpin siRNA expression cassettes targeting FUT8 and GMD (D). The transcribed shRNAs and tRNA-shRNA fusion product were processed into siRNAs by Dicer.