Table 3.

Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with the FUT8 and GMD siRNA tandem expression vector

Clone Name	siRNAa	Relative composition of monosaccharide			Fucose(-)%c
		Fucoseb	GlcNAc	Mannose
32-05-12	-	0.96	4.00	2.60	4
FG1	+	n.d.	4.00	2.58	100
FG3	+	n.d.	4.00	2.65	100
FG4	+	n.d.	4.00	2.63	100
FG7	+	0.02	4.00	2.57	98
FG9	+	n.d.	4.00	2.61	100
FG13	+	0.02	4.00	2.55	98
FG16	+	n.d.	4.00	2.57	100
FG20	+	n.d.	4.00	2.62	100

^aIntroduction of FUT8 and GMD siRNA tandem expression vector.

^bMolar ratios calculated vs. 4 GlcNAc; n.d., not detectable.

^cTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-b) × 100.