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. 2008 Feb 13;3(2):e1557. doi: 10.1371/journal.pone.0001557

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Chao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Monocytes were infected with DENV-2 New Guinea C (NGC) and 16681 strains at an MOI of 0.1 for five days. The virus titer in the cell culture supernatants were analyzed by plaque assay (—) and the intracellular replication of virus in the infected cells were detected by flow cytometry ( ---- ).