Figure 4.

Functional coupling of the five-TM chemokine receptors to PTX-sensitive G proteins. HEK293 cells were transiently transfected the wild-type or mutant chemokine receptors and pretreated with or without (control) 100 ng/ml PTX for 24 hr (A). The cells were transfected with the indicated chemokine receptor alone (control) or cotransfected with Giα2 (B) or Goα1 (C). The basal GTPγS binding was 1.08 ± 0.05 nmol/mg protein. Data presented are averages and error ranges of two separate experiments performed in duplicate. ∗∗, P < 0.01 compared with controls.