Skip to main content
. 2007 Oct 22;196(1):149–158. doi: 10.1677/JOE-07-0391

Figure 2.

Figure 2

Representative immunoblots showing the effects of (A) Lys-γ3-MSH and (B) ACTH on the phosphorylation state of HSL at specific serine residues in 3T3-L1 adipocytes. The phosphorylation state of HSL was determined, after a 20-min stimulation in the presence or absence of Lys-γ3-MSH or ACTH at increasing dose from 1 pM to 100 nM, by analysing the degree of phosphorylation of serine residues 563, 565 and 660 using antibodies specific for each phosphorylated serine site. (0), no peptide added. Densitometric analysis was performed on the results of three independent experiments and is presented as the means of the phosphorylated HSL content divided by the mean of the total HSL content for the same sample. The error bars represent the mean±s.e.m. *P<0·05 and **P<0·01 indicate a significant difference from the corresponding untreated value.