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. 2007 Oct 22;196(1):149–158. doi: 10.1677/JOE-07-0391

Figure 3.

Figure 3

(A) Analysis of the effect of Lys-γ3-MSH, ACTH, α-MSH and NDP-α-MSH on the phosphorylation state of perilipin A in 3T3-L1 adipocytes. The phosphorylation state of perilipin A was determined by analysing the migratory rate of perilipin A, on 6·5% SDS-PAGE gels, either 62 kDa (unstimulated) or 67 kDa (stimulated, hyperphosphorylated). (−), no peptide added. (0), time point zero. (B) Densitometric analysis of the effect of Lys-γ3-MSH, ACTH, α-MSH and NDP-α-MSH on the phosphorylation state of perilipin A in 3T3-L1 adipocytes. The phosphorylation state of perilipin A was determined by analysing the migratory rate of perilipin A, on 6·5% SDS-PAGE gels, either 62 kDa (unstimulated) or 67 kDa (stimulated, hyperphosphorylated). All results presented, except those at time point 0 (0) and (−) (no peptide added), are the average of three responses from independent experiments. Results presented at time point (0) and (−) are the average of 12 responses from three independent experiments. The error bars represent the mean ±s.e.m. (a) (P=0·0194) is significantly different from the control (−) value. (b) (P=0·0022) is significantly different from the control (−) value. (c) (P=0·0061) is significantly different from the control (−) value. (d) (P=0·004) is significantly different from the control (−) value. *(P<0·0001) indicates that values are significantly different from the control (−) value.