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. 1999 Jul 6;96(14):7932–7937. doi: 10.1073/pnas.96.14.7932

Figure 2.

Figure 2

Interaction of CD44 and TCR with PS1 in vitro. (A) HEp2 cells. Western blot analysis of cell proteins bound to immobilized recombinant PS1 with mAb to CD44 (HEp-2 cells). Lanes: 1, cell extract; 2, unbound fraction; 3, last wash; 4, elution with thrombin. (B) Jurkat cells. Western blot analysis of cell proteins bound to immobilized recombinant PS1 with mAb to CD44. Lanes: 1, cell extract; 2, unbound fraction; 3, last wash; 4, elution with thrombin. (C) Jurkat cells. Western blot analysis of cell proteins bound to immobilized recombinant PS1 with mAb to TCR. Lanes: 1, cell extract; 2, unbound fraction; 3, last wash; 4, elution with thrombin. (D) Detection of PS1 in CD44 and TCR immunoprecipitates from Jurkat cells. Western blot analysis with PS1-N antibody. Lanes: 1, cell extract; 2, immunoprecipitation with anti-CD44 antibody; 3, immunoprecipitation with anti-TCR antibody.