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Scanning immunoelectron micrographs of Jurkat cells adherent to a collagen matrix. After overnight adhesion to collagen, cells were incubated with mAb to TCR (A), CD44 (B), and PS1-N (C– G) and with affinity-purified rabbit polyclonal antibody to transthyretin (H). Bound antibodies were visualized with 5-nm gold-labeled antibody to mouse IgM (TCR), mouse IgG (CD44), and rabbit IgG to PS1-N and transthyretin followed by silver enhancing. (Bar = 11.258 μm; ×3,500.)
