Figure 4.

The amount of NMDA receptors in cerebellum is lower in mature male rats than in young male rats. Thirty micrograms of protein from homogenates of the cerebellum from 3-mo-old or 7-mo-old rats was subjected to SDS–polyacrylamide gel electrophoresis using an 8% acrylamide gel. The NR1 subunit was visualized by immunoblotting as indicated in Materials and Methods. Typical immunoblotting is shown for males (B) and females (C). The intensities of the bands were quantified and are shown in A. Values are the mean ± SEM of five rats per group and are expressed as percentage of NR1 amount in 3-mo-old rats. Data of different sex and age were analyzed using two-way ANOVA. There were no significant differences between sexes, but the effect of age is significant (P < 0.05). Values that are significantly different from young (3-mo-old) rats are indicated by asterisks: *P < 0.05 (two-way ANOVA). Pooled data were analyzed using Student’s t-test. Significant difference from young (3-mo-old) rats is indicated by asterisks: *P < 0.05.