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. 1999 Jul 6;96(14):7938–7943. doi: 10.1073/pnas.96.14.7938

Figure 6.

Figure 6

Nuclear import of cyclin B1–Cdc2 by importin β is Ran-independent and energy-dependent. Nuclear import reactions were performed with cyclin B1ΔCRS–Cdc2K− (A–E), wild-type cyclin B1–Cdc2 (F–J), or FITC-NLS-BSA (K and L) in the absence (A, F, and K) or presence of 2.7 μM importin β (B–D, G–I, and L) or importin β-(71–876) (E and J). (C and H) Ran Q69L (2 μM) was included in the reaction. (D and I) Hexokinase (0.4 unit/μl) and glucose (5 mM) were included to deplete residual ATP. Cells were analyzed by direct immunofluorescence (K and L) or secondary immunofluorescence with monoclonal anti-cyclin B1 antibodies (A–J).