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. 2006 Apr;168(4):1375–1384. doi: 10.2353/ajpath.2006.050989

Table 2.

Primer Sequences and Restriction Enzymes for the MSP and the COBRA Analysis

MINT clones Forward primer Reverse primer Restriction enzyme
MINT 1 F 5′ GGGTTGGAGAGTAGGGGAGTT 3′ R 5′ CCATCTAAAATTACCTCRATAACTTA 3′ TaqI
MINT 25 F 5′ TYGGTGTTTGTAAAGGGTTGGAAT 3′ R 5′ CCCRAACTAAAAACTAACTCRTAA 3′ RsaI
MINT 31 F 5′ GAYGGYGTAGTAGTTATTTTGTT 3′ R 5′ CATCACCACCCCTCACTTTAC 3′ MaeII
Gene promoters
p16-M F 5′ TTATTAGAGGGTGGGGCGGATCGC 3′ R 5′ GACCCCGAACCGCGACCGTAA 3′
p16-U F 5′ TTATTAGAGGGTGGGGTGGATTGT 3′ R 5′ CAACCCCAAACCACAACCATAA 3′
Cox2-M F 5′ TTAGATACGGCGGCGGCGGC 3′ R 5′ TCTTTACCCGAACGCTTCCG 3′
Cox2-U F 5′ ATAGATTAGATATGGTGGTGGTGGT 3′ R 5′ CACAATCTTTACCCAAACACTTCCA 3′
GSTP1-M F 5′ TTCGGGGTGTAGCGGTCGTC 3′ R 5′ GCCCCAATACTAAATCACGACG 3′
GSTP1-U F 5′ GATGTTTGGGGTGTAGTGGTTGTT 3′ R 5′ CCACCCCAATACTAAATCACAACA 3′
RASSF1A-M F 5′ GTGTTAACGCGTTGCGTTGCGTATC 3′ R 5′ AACCCCGCGAACTAAAAACGA 3′
RASSF1A-U F 5′ TTTGGTTGGAGTGTGTTAATGTG 3′ R 5′ CAAACCCCACAAACTAAAAACAA 3′
E-cadherin-M F 5′ TTAGGTTAGAGGGTTATCGCGT 3′ R 5′ TAACTAAAAATTCACCTACCGAC 3′
E-cadherin-U F 5′ TAATTTTAGGTTAGAGGGTTATTGT 3′ R 5′ CACAACCAATCAACAACACA 3′
APC-M F 5′ TATTGCGGAGTGCGGGTC 3′ R 5′ TCGACGAACTCCCGACGA 3′
APC-U F 5′ GTGTTTTATTGTGGAGTGTGGGTT 3′ R 5′ CCAATCAACAAACTCCCAACAA 3′