Table 4.

Promoter Methylation Status and its Clinicopathological Correlations in 60 HCCs

CpG island	Methylation status	No. of samples	Gender			Age (average)	P value**	Viral infection				Histological differentiation
			Male	Fe- male	P value*			HBV- posi- tive	HCV- posi- tive	Nega- tive	P value***	Well	Mode- rate	Poor	P value***
MINT1	Methylated	13	9	4	0.47	61.23	0.39	3	9	1	0.24	1	6	6	0.94
	Unmethylated	47	37	10		58.81		14	21	12		5	20	22
MINT25	Methylated	3	3	0	0.33	57.33	0.40	0	3	0	0.21	1	0	2	0.19
	Unmethylated	57	43	14		59.44		17	27	13		5	26	26
MINT31	Methylated	40	29	11	0.28	58.68	0.56	13	24	3	0.00080	6	15	19	0.14
	Unmethylated	20	17	3		60.65		4	6	10		0	11	9
p16	Methylated	39	29	10	0.56	62.05	0.027	9	26	4	0.00092	5	18	16	0.40
	Unmethylated	21	17	4		54.29		8	4	9		1	8	12
Cox2	Methylated	23	18	5	0.82	58.13	0.52	7	11	5	0.95	2	9	12	0.80
	Unmethylated	37	28	9		60.08		10	19	8		4	17	16
GSTP1	Methylated	32	21	11	0.031	58.34	0.47	11	18	3	0.045	3	16	13	0.53
	Unmethylated	28	25	3		60.46		6	12	10		3	10	15
RASSF1A	Methylated	45	36	9	0.29	60.67	0.20	13	26	6	0.019	5	18	22	0.65
	Unmethylated	15	10	5		55.33		4	4	7		1	8	6
E-cadherin	Methylated	26	20	6	0.97	59.23	0.95	5	15	6	0.38	3	13	10	0.54
	Unmethylated	34	26	8		59.41		12	15	7		3	13	18
APC	Methylated	46	42	4	0.19	60.79	0.13	15	28	10	0.31	6	24	23	0.33
	Unmethylated	14	11	3		48.29		2	2	3		0	2	5

			Intrahepatic metastasis			Vascular invasion			TNM classification			Maximum diameter
			Posi- tive	Nega- tive	P value*	Posi- tive	Nega- tive	P value*	I to II	III to IV	P value*	<5.0 cm	5.0 cm<	P value*
MINT1	Methylated	13	7	6	0.56	9	4	0.30	8	5	0.45	9	4	0.53
	Unmethylated	47	21	26		25	22		34	13		28	19
MINT25	Methylated	3	1	2	0.63	2	1	0.44	2	1	0.90	2	1	0.85
	Unmethylated	57	27	30		25	32		40	17		35	22
MINT31	Methylated	40	17	23	0.36	23	17	0.85	29	11	0.55	28	12	0.060
	Unmethylated	20	11	9		11	9		13	7		9	11
p16	Methylated	39	16	23	0.23	18	21	0.025	32	7	0.0055	29	10	0.91
	Unmethylated	21	12	9		16	5		10	11		8	3
Cox2	Methylated	23	13	10	0.23	14	9	0.60	13	10	0.072	15	8	0.66
	Unmethylated	37	15	22		20	17		29	8		22	15
GSTP1	Methylated	32	13	19	0.32	17	15	0.55	25	7	0.14	23	9	0.082
	Unmethylated	28	15	13		17	11		17	11		14	14
RASSF1A	Methylated	45	21	24	1.00	25	20	0.76	34	11	0.10	30	15	0.17
	Unmethylated	15	7	8		9	6		8	7		7	8
E-cadherin	Methylated	26	13	13	0.65	15	11	0.89	20	6	0.31	18	8	0.29
	Unmethylated	34	15	19		19	15		22	12		19	15
APC	Methylated	46	24	29	0.55	28	25	0.099	39	14	0.095	35	18	0.055
	Unmethylated	14	4	3		6	1		3	4		2	5

^*P values were calculated by χ² test for 2 × 2 squares. 

^**P values were calculated by Student unpaired t-test. 

^***Pvalues were calculated by χ² test for 3 × 2 squares.