Figure 5.

Simulation and time–space tracking of vesicles under experimental dynamic conditions determined for vesicular membrane traffic (see Fig. 4A and Table 1). (A) Three sections of a series of forty simulated sections at indicated time steps. (B) Visualization of trajectories within the Open Inventor Scene Viewer. Accurately matched trajectories are color-encoded whereas unmatched trajectories are visualized in gray. Note that, despite several intersections of vesicles trafficking at high speed (arrows), the vast majority of trajectories was accurately found by the automated object tracking module (see also Fig. 4B). (See the supplemental data on the PNAS web site, www.pnas.org.)