Table 1.

Simulation and time-space tracking of vesicles

Simulation	vstart, pixel/frame	vmax, pixel/frame	a, pixel/frame2
1	1	2	1	2
2	5	10	1	9
3	5	10	6	2
4	5	10	7	15
5	5	10	8	28
6	5	10	0 ≤ acc ≤ 6	3

Six simulations were performed for different parameter settings of initial velocity (v_start), maximal allowed velocity (v_max), and acceleration (a). The relative deviation between average velocities of simulated trajectories (v_sim) and automatically tracked trajectories (v_auto) is given in the fourth row.