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. 1999 Jul 6;96(14):7956–7961. doi: 10.1073/pnas.96.14.7956

Figure 1.

Figure 1

Phenotypes associated with the expression of Bax and Bax deletion mutants in plants. (A) Schematic diagrams of the TMV vector constructs carrying the GFP and Bax cDNAs (not drawn to scale). Open boxes represent viral ORFs with the sizes of the encoded proteins indicated in kDa; CP is the TMV coat protein; filled boxes show the GFP and Bax ORFs. Schematic diagrams of the Bax and Bax deletion mutant ORFs are shown below. (B) Comparison of the localized HR induced by TMV.GFP on N. edwardsonii with symptoms induced by TMV.Bax, the Bax deletion mutants, and TMV.GFP on N. benthamiana at 4 dpi. The Bax deletion mutants used as inoculum are indicated by the deletion of the prefix TMV.Bax from the plasmid name. (C) Western blot analysis of protein accumulation in N. benthamiana leaves 2, 3, and 4 dpi with TMV.Bax, TMV expressing the Bax deletion mutants and mock inoculated plants. Blots were probed with antiserum raised against either Bax or the TMV CP.