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. 1997 Jun 1;109(6):731–740. doi: 10.1085/jgp.109.6.731

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Effects of pH_p and pH_o on heating-activated outward current. (A) Averaged I-V plots with pH_o 7.3 at pH_p 5.5 (squares, n = 9), 6.6 (triangles, n = 3), and 7.3 (circles, n = 7). The current was measured at the end of 500-ms voltage pulses. The internal buffer (Mes) was 10 mM in seven of nine cells at pH_p 5.5 and 120 mM in two cells at pH_p 5.5 and in three cells at pH_p 6.6. B, I-V relations obtained from voltage ramp applied at 0 mV when the pH_o was changed from 7.3 (1) to 8.7 (2) and then returned to 7.3 (3). The internal medium contained K-glutamate. (C) I-V relations recorded with the pipette solution containing CsCl when the pH_o was increased from 7.3 to 9.0. B and C were obtained from different cells at pH_p 5.5 with 10 mM Mes. Leak current was subtracted. A–C were obtained at 32°C.