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. 1997 Jan 1;109(1):53–60. doi: 10.1085/jgp.109.1.53

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Effect of ATP on [Ca²⁺]_i in CK1.4 cells. Cells were loaded with fura-2, preincubated for 1 min in Na-PSS + 1 mM CaCl₂ and diluted 30-fold into cuvettes containing Na- or Li-PSS plus 0.3 mM EGTA. ATP (0.3 mM) was added to the cuvette at 30 s in each panel and 1 mM CaCl₂ was added at various intervals after ATP as indicated in the individual panels; in A, ATP and CaCl₂ were added simultaneously and in B, C, and D, CaCl₂ was added 60, 120, and 180 s after ATP. In E, ATP was not added and CaCl₂ was added at 150 s. Fura-2 fluorescence was monitored with alternate excitation at 340 and 380 nm. For all experiments, n = 3–4 except B, where n = 5–6.