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. 1997 Jan 1;109(1):41–51. doi: 10.1085/jgp.109.1.41

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Ba²⁺ influx in CK1.4 cells detected with fura-2. Cells were loaded with fura-2 without (A) or with (B) 0.4 mM ouabain, washed, and preincubated for 1 min in Na-PSS + 1 mM CaCl₂ and then diluted 30-fold into cuvettes containing either Li-PSS or Na-PSS, as indicated; 0.3 mM EGTA was included in both media. BaCl₂ (1 mM) and EGTA (10 mM) were added as indicated by the arrows (n = 5).