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. 1997 May 1;109(5):555–570. doi: 10.1085/jgp.109.5.555

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Binding isotherms at 0°C and pH 7.7 for binding of ADP to Na,K-ATPase in (A) NaCl-, (B) Na₂SO₄-, and (C) NaNO₃-solutions of increasing concentration. In each panel, the anion concentration (and thereby the ionic strength) increases from the top-curve (1) to the bottom-curve (7 or 8), see Table I. In panel A is also shown a binding isotherm in 100 mM Na-acetate (□). The concentrations of bound and free ADP are those in the assay solutions, so that the B_max value in each series depends upon the (constant) concentration of enzyme-preparation used for the assays of that series—for further details see materials and methods. The relative error on F due to pipetting and counting is ±2% and that on B is about ±3% (see also the double determinations at the highest B values). The curves are the best fit by nonlinear regression (data weighted by B⁻², see Jensen et al., 1984) to a one-site model: B = B_max · F/(K _diss + F), and the values for B_max and K _diss derived from these data are given in Table I.

Binding isotherms at 0°C and pH 7.7 for binding of ADP to Na,K-ATPase in (A) NaCl-, (B) Na₂SO₄-, and (C) NaNO₃-solutions of increasing concentration. In each panel, the anion concentration (and thereby the ionic strength) increases from the top-curve (1) to the bottom-curve (7 or 8), see Table I. In panel A is also shown a binding isotherm in 100 mM Na-acetate (□). The concentrations of bound and free ADP are those in the assay solutions, so that the B_max value in each series depends upon the (constant) concentration of enzyme-preparation used for the assays of that series—for further details see materials and methods. The relative error on F due to pipetting and counting is ±2% and that on B is about ±3% (see also the double determinations at the highest B values). The curves are the best fit by nonlinear regression (data weighted by B⁻², see Jensen et al., 1984) to a one-site model: B = B_max · F/(K _diss + F), and the values for B_max and K _diss derived from these data are given in Table I.