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. 1997 May 1;109(5):589–605. doi: 10.1085/jgp.109.5.589

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Single-channel records and ensemble averages of WT and F1489 mutant channels. Traces of single-channel activity and ensemble averages (last trace in each column) from cell-attached patches. The arrows indicate the beginning of 40-ms depolarizations to −20 mV from a holding potential of −140 mV. The vertical calibration bar is 1 pA for single-channel traces and 0.5 pA for ensemble averages. The number of channels in the patches for the single-channel traces shown were two for WT and one for each mutant. The dotted ensemble averages are normalized WT data for comparison.