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. 1997 Mar 1;109(3):401–414. doi: 10.1085/jgp.109.3.401

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High time-resolution traces showing the onset of macroscopic current inhibition by 100 μM PB. The trace labeled “control” was obtained in the absence of PB. The trace labeled “equilibrium” was obtained when both normal (agonist-free) and test (agonist-containing) solutions contained 100 μM PB. The trace labeled “onset” was obtained when only the test solution contained PB. The close similarity between the “equilibrium” and “onset” traces indicates that PB does not interact strongly with closed channels. 300 μM ACh, −50 mV.