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. 1997 Mar 1;109(3):345–360. doi: 10.1085/jgp.109.3.345

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AE1 E699Q-associated currents. (A) Mean I-V curves and representative current traces from Na₂SO₄-loaded oocytes in extracellular Cl⁻ medium expressing wt AE1 (filled squares, n = 20 oocytes from 6 frogs) and AE1 E699Q (filled circles, n = 54 oocytes from 8 frogs). *P < 0.04; NS, P > 0.05. (B) Comparison of E699Q-mediated currents in Na₂SO₄-loaded oocytes in the presence of extracellular Cl⁻ (filled circles), extracellular isethionate (open squares, n = 44 oocytes from 7 frogs), and extracellular sulfate (open triangles, n = 5 oocytes from 1 frog). *P < 0.002. (C) Comparison of E699Q-mediated currents in Na₂SO₄-loaded oocytes in extracellular chloride in the absence (filled circles) and presence of 200 μM DNDS (open diamonds, n = 24 oocytes from 4 frogs) *P < 0.03. Voltage was stepped from a holding potential of −50 mV to test potentials between −80 and +60 mV in 20-mV increments. At potentials ≥+80 mV, endogenous outward currents were activated in all groups of oocytes. Data recorded in extracellular chloride from AE1 E699Q-expressing oocytes is replicated in all three panels.

AE1 E699Q-associated currents. (A) Mean I-V curves and representative current traces from Na₂SO₄-loaded oocytes in extracellular Cl⁻ medium expressing wt AE1 (filled squares, n = 20 oocytes from 6 frogs) and AE1 E699Q (filled circles, n = 54 oocytes from 8 frogs). *P < 0.04; NS, P > 0.05. (B) Comparison of E699Q-mediated currents in Na₂SO₄-loaded oocytes in the presence of extracellular Cl⁻ (filled circles), extracellular isethionate (open squares, n = 44 oocytes from 7 frogs), and extracellular sulfate (open triangles, n = 5 oocytes from 1 frog). *P < 0.002. (C) Comparison of E699Q-mediated currents in Na₂SO₄-loaded oocytes in extracellular chloride in the absence (filled circles) and presence of 200 μM DNDS (open diamonds, n = 24 oocytes from 4 frogs) *P < 0.03. Voltage was stepped from a holding potential of −50 mV to test potentials between −80 and +60 mV in 20-mV increments. At potentials ≥+80 mV, endogenous outward currents were activated in all groups of oocytes. Data recorded in extracellular chloride from AE1 E699Q-expressing oocytes is replicated in all three panels.