Table II.
Electrophysiological and Morphological Data from Oocytes Expressing Connexin50
Oocyte* | Current | Conductance | Capacitance | Hemichannel density | ||||
---|---|---|---|---|---|---|---|---|
nA‡ | μS § | nF | μm−2 ∥ | |||||
Control | 5 | 0.2 | 363 | 0 | ||||
6 | −10 | 0.4 | ND | BDL¶ | ||||
24 | −415 | 16.6 | 355 | 195 ± 13 | ||||
48 | −840 | 33.6 | 350 | 407 ± 36 | ||||
72 | −1160 | 46.4 | 313 | 537 ± 47 | ||||
96 | −865 | 34.6 | 261 | 442 ± 69 |
The duration of incubation after cRNA injection is given in hours.
The whole-cell current was measured at −50 mV and 10 μM [Ca2+]0, and was corrected for the background oocyte current.
The whole-cell conductance was measured as a chord conductance at −50 mV.
The density of hemichannels (mean ± SEM) (excluding those in plaques) corresponds to the density of P face particles after correction for the density of the endogenous particles.
BDL, below detection level.