Figure 1.
(a) Apoptosis is reduced on UVB exposure at low temperature. HeLa cells were UVB-irradiated (400 J/m2) at either 37°C or after being kept at 4°C for 10, 20, or 30 min. After irradiation, cells were kept on ice for 5 min and then incubated at 37°C for 16 hr. Control cells were not UVB-exposed but were incubated at 4°C for 30 min or kept at 37°C. (b) Cells were incubated with different concentrations of cis-platin either at 37°C or kept for 30 min before and 5 min after cis-platin stimulation. Apoptosis was evaluated 16 hr later with a cell death ELISA. Rate of apoptosis is reflected by the enrichment of nucleosomes in the cytoplasm shown on the y axis (mean ± SD of triplicate samples). Data presented show one representative of two independently performed experiments.