Figure 2.

Enhancement of DNA repair reduces UVB-induced apoptosis. After UVB irradiation, increasing amounts of Photosomes were applied to the medium and cells were incubated at 37°C for 1 hr. Subsequently, photoreactivation was carried out by irradiating cells with PRL. For control purposes, cells were either left untreated, irradiated with PRL or UVB alone, or with UVB and PRL in the absence of Photosomes. In addition, cells were incubated with empty liposomes (lip) followed by exposure to PRL. After 16 hr at 37°C, apoptosis was evaluated with a cell death ELISA. Enrichment factor was used as a parameter of apoptosis and is given as the mean ± SD of three independently performed experiments.