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. 2000 Mar 1;115(3):287–304. doi: 10.1085/jgp.115.3.287

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Summary data showing the effect of ATP and cytosolic fraction on channel activity and open time constants. (A) Channel activity (NP_o) was determined at various patch conditions. (B) Open time constants were calculated from the rate constants (τ_o1 = 1/k ₋₄ and τ_o2 = 1/k ₋₃) obtained by fitting the data to the two kinetic schemes. The kinetic scheme in dotted lines was used to analyze channel kinetics when currents were recorded with GTP or cytosolic fraction. 5 mg/ml protein cytosolic fraction was used for all experiments. Each bar shows the mean ± SD of four values. *Significant difference from the value of the first bar (P < 0.05).