Table 5.
Patch-to-Patch Variation in the Kinetic-Parameter Estimates of δS→T AChR Unliganded Gating
| Rates | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Patch | k 01 | k 10 | k 02 | k 20 | k 03 | k 30 | k 34 | k 43 | n |
| s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | ||
| 1 | 37.5 ± 1.8 | 6547 ± 335 | 9.2 ± 1.5 | 1416 ± 229 | 2.8 ± 0.4 | 111.8 ± 13.5 | 53.3 ± 7.5 | 2683 ± 436 | 4226 |
| 2 | 35.2 ± 1.5 | 6263 ± 217 | 3.2 ± 1.1 | 1827 ± 814 | 1.5 ± 0.5 | 240.2 ± 46.6 | 44.0 ± 10.0 | 2054 ± 1147 | 6317 |
| 3 | 6.8 ± 0.8 | 10057 ± 1164 | 2.9 ± 0.8 | 2975 ± 460 | 0.17 ± 0.06 | 52.7 ± 17.8 | 19.3 ± 10.8 | 3254 ± 1874 | 802 |
| 4 | 5.1 ± 0.7 | 13267 ± 2244 | 1.5 ± 0.5 | 3739 ± 707 | 0.18 ± 0.07 | 164.2 ± 66.9 | 138.8 ± 50.1 | 1980 ± 822 | 462 |
Fig. 3 A was used as the kinetic model. The smaller number of intervals detected in patches 3 and 4 mainly resulted from the slower opening rates k 01, k 02, and k 03. Variation of these values between patches reflects differences in both the opening rate constants and the number of channels in the patch. The total duration of the recordings in the four cases were similar but not equal. Dead times were (μs): 18, 45, 44, and 46 for patches 1–4, respectively. Standard errors were calculated from the curvature of the likelihood surface at its maximum. n, number of intervals.