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. 2001 Feb 1;117(2):165–180. doi: 10.1085/jgp.117.2.165

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Intersubunit proximities and the distribution of spin–spin dipolar couplings at the COOH terminus of KcsA. (A) CW-EPR spectra of residues showing strong spin–spin interactions. A comparison is made between fully labeled channels (a maximum of four spin/channel), in red, and channels that show no spin–spin interaction by virtue of being underlabeled at a 1:10 label/channel molar ratio (so that on average they have 1 spin/channel), in black. (B) Spin–spin interaction parameter (Ω) calculated for the all residues of the COOH terminus. As the Ω parameter serves as a gross estimate of intersubunit proximity, this particular pattern of per-residue Ω has been taken to indicate the formation of a four-helix bundle. The gray bars represent charged residues.