Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Feb 1;117(2):103–118. doi: 10.1085/jgp.117.2.103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Fluorescence analysis of membrane retrieval. (A) Confocal image of two oocytes labeled with 10 μM tetramethylrhodamine-5-maleimide, a membrane-impermeant thiol-reactive fluorophore. The oocyte at left was injected with v-Src and Kir2.1-Y242TAG suppressed with Tyr(ONB). The oocyte on the right was not injected. (B) Fluorescence measurements for individual oocytes coexpressing v-Src and Kir2.1 Y242Tyr(ONB) and exposed to PAO. Oocytes were labeled with 10 μM tetramethylrhodamine-5-maleimide for 30 min before recording. Fluorescence is measured as PMT output voltage. As indicated, controls included omission of coinjected v-Src and omission of irradiation. (C) Normalized, average fluorescence change at t = 30 min for oocytes expressing Kir2.1-Y242TAG suppressed with Tyr(ONB) and treated with PAO, and then labeled with tetramethylrhodamine maleimide. The first column represents a control in which oocytes were not coinjected with v-Src. In all other columns, oocytes expressed the Kir2.1-Y242Tyr(ONB) along with v-Src. The second column represents a control in which oocytes were not irradiated. In the third column, no exogenous dynamin was added. The fourth column shows oocytes coexpressing WT dynamin. The fifth column represents oocytes coexpressing dynamin-K44A. Each bar represents six or seven oocytes. The average values (and ranges) for 100% fluorescence were 5.76 (3–9.5), 5.74 (3.5–7.2), 6.45 (3.7–9.5), 5.60 (3.93–6.92), and 5.62 (1–9.7) V.