Figure 4.
The exon B-encoded, leucine-rich sequence of p120ctn is necessary and sufficient to direct export of microinjected OV from the nucleus to the cytoplasm. A mixture of peptide-conjugated OV and FITC-BSA was injected into the nuclei of L929 cells. Either a wild-type NES sequence (a and b) or a mutated NES sequence (c and d) was conjugated to OV. At 20 min after injection, cells were fixed with 3% paraformaldehyde and stained with anti-OV antibody and Alexa594-conjugated secondary antibody. Dual-channel fluorescence was used to reveal both OV (a and c) and FITC-BSA (b and d) in the injected cells. (Bar = 10 μm.)