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. 2003 Jan;121(1):3–16. doi: 10.1085/jgp.20028671

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Fluo-3 relative fluorescence variation in an ROI from GLT cells: GLT-NEO, GLT-α₁, and GLT-α₁ in the presence of nifedipine and ryanodine. (A) Relative fluorescence variation in an ROI during the depolarization-evoked stimulus (47mM K⁺) in previously fluo-3 a.m.–loaded GLT cells. Mock-transfected GLT (open squares); GLT-α₁–transfected (filled triangles), and GLT-α₁–transfected cells plus nifedipine (filled circles). (B) Relative fluorescence variation in an ROI during the depolarization evoked stimulus (47 mM K⁺) in previously fluo-3 a.m.–loaded GLT cells: GLT-α₁–transfected, (filled triangles), GLT-α₁–transfected cells plus ryanodine (10 μM) (filled circles), and GLT-α₁–transfected cells plus nifedipine and ryanodine (open triangles). All these experiments were carried in the presence of 0.5 mM EGTA to reduce any Ca²⁺ entry from the medium.