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. 2003 Jan;121(1):3–16. doi: 10.1085/jgp.20028671

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Nifedipine inhibits K⁺ depolarization–induced increases in c-fos and c-jun transcription. (A) Myotubes were pretreated for 30 min with ethanol (vehicle for nifedipine) or with 10 μM nifedipine under resting conditions and depolarized with 84 mM potassium in a medium containing ethanol or nifedipine. The levels of c-fos and c-jun mRNAs were determined by Northern blot analysis of RT-PCR products. The results from both methods were normalized to GAPDH expression and presented as mean ± SE (n = 4–5) of the fold-induction effect of depolarization with respect to the control (no depolarization). (B) Myotubes were pretreated for 30 min with 20 μM ryanodine and depolarized in the presence of ryanodine. The levels of c-fos and c-jun mRNAs were determined in three experiments by RT-PCR. The results obtained at 15 min of exposure to high potassium in both cases are presented. Paired t tests were performed. *, P < 0.05; ***, P < 0.001.